Abstract: To resolve the frequent occurrence of root rot in Salvia miltiorrhiza Bunge under forest plantations in Shangluo, Shaanxi Province, China, this study isolated pathogenic fungi from infected roots using tissue isolation. The pathogenic fungi were identified by morphological and molecular biological methods, and the effect of biocontrol strain DSSR-2 Bacillus velezensis Ruiz-García. on disease resistance and growth promotion was evaluated. Fusarium oxysporum Schltdl. was identified as the primary pathogen causing root rot in Salvia miltiorrhiza Bunge. under forest plantations in Shangluo Shaanxi province. The biocontrol strain DSSR-2 was identified as Bacillus velezensis Ruiz-García.. Plate confrontation assays demonstrated that DSSR-2 exhibits significant antagonistic activity against eight plant pathogenic fungi, including F. oxysporum, with inhibition rates ranging from 80.56% to 97.56%. Additionally, this strain possesses plant growth-promoting traits, evidenced by its ability to produce indole-3-acetic acid （IAA）, siderophores, and phosphate-solubilizing enzymes. DSSR-2 fermentation broth achieved a 65.56% control efficacy against S. miltiorrhiza root rot and significantly increased leaf length, leaf width, and fresh weights of both aboveground and underground parts. Physiological and biochemical analyses revealed that DSSR-2 enhances the activities of superoxide dismutase （SOD） and peroxidase （POD） in the roots of S. miltiorrhiza, reduces malondialdehyde （MDA） content, and thereby alleviates oxidative damage. This study confirms that B. velezensis DSSR-2 possesses dual beneficial effects of disease suppression and growth promotion. It provides both a theoretical basis and technical support for the green control of S. miltiorrhiza root rot and the sustainable development of integrated forest-medicinal plant systems.